ABSTRACT
Auto-fluorescence bronchoscopy [AFB] has been used for the identification and localization of intra-epithelial pre-neoplastic and neoplastic lesions within the bronchus. To determine the applicability of AFB for the detection and localization of precancerous and cancerous lesions, in addition to analyzing the morphologic presentation, their association to histological type and the variation between genders. A five-year study involving 4983 patients, who underwent routine bronchoscopy [B] examination in a local tertiary teaching hospital, was done. The B examination was performed under intratracheal lidocaine, and samples were obtained using suitable approach. One thousand four hundred and eighty-five pathologically confirmed lung cancer patients were included in the study. The following parameters were studied: Morphological presentation, biopsy sites, histology. Differences between the groups were analyzed using Chi square test. One thousand four hundred and eighty-five patients who had hyperplasia or neoplastic lesions were further confirmed as lung cancer pathologically. Lung cancer was more commonly found in the right lung [51.58% vs. 42.82%]. The lesion occurred more frequently in the upper lobe than the lower lobe [44.17% vs. 22.42%]. Male patients with squamous cell carcinoma showed upper lobe involvement more commonly, while the left main bronchus was more commonly involved in female patients. Adenocarcinoma mostly involved lesion of the upper lobe. Squamous cell carcinoma and small cell carcinoma were the major proliferative types [80.15% and 76.16% respectively]. AFB is efficient in the detection of pre-invasive and invasive lung lesions. The morphological presentation is associated to the histological type. There is variation in the presentation and histology of cancerous lung lesions between genders
Subject(s)
Humans , Male , Female , Bronchoscopy/methods , Fluorescence , Neoplasm InvasivenessABSTRACT
Objective To explore the effect and significance of AQP4 mRNA expression in bronchial epithelium of chronic obstructive pulmonary disease (COPD) on airway inflammation. Methods Bronchial epithelium was obtained by bronchoscopy in patients with COPD (n=30) and healthy controls (n=30) whose bronchial epithelium was confirmed to be normal by the pathologist. AQP4 mRNA were studied by RT-PCR method. Inflammatory cells infiltration was observed by HE staining; pulmonary function test was performed too before bronchoscopy. Results Compared with that in the healthy controls, AQP4 mRNA expression in COPD was reduced (P